Genetic transformation of Brassica nigra by agrobacterium based vector and direct plasmid uptake

Gupta, Vibha and Sita, Lakshmi G and Shaila, MS and Jagannathan, V (1993) Genetic transformation of Brassica nigra by agrobacterium based vector and direct plasmid uptake. In: Plant Cell Reports, 12 (7-8). pp. 418-421.

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Abstract

Genetic transformation systems have been established for Brassica nigra (cv. IC 257) by using an Agrobacterium binary vector as well as by direct DNA uptake of a plasmid vector. Both the type of vectors carried nptII gene and gus gene. For Agrobacterium mediated transformation, hypocotyl tissue explants were used, and up to 33% of the explants produced calli on selection medium. All of these expressed B-glucuronidase gene on histochemical staining. Protoplasts isolated from hypocotyl tissues of seedlings could be transformed with a plasmid vector by FEG mediated uptake of vector DNA. A number of fertile kanamycin resistant plants were obtained using both the methods, and their transformed nature was confirmed by Southern blot analysis and histochemical staining for GUS. Backcrossed and selfed progenies of these transformed plants showed the presence of npt and gus genes.

Item Type:	Journal Article
Publication:	Plant Cell Reports
Publisher:	Springer
Additional Information:	Copyright of this article belongs to Springer.
Keywords:	Transformation;Agrobacterium;Direct gene transfer.
Department/Centre:	Division of Biological Sciences > Microbiology & Cell Biology
Date Deposited:	10 Feb 2011 05:36
Last Modified:	10 Feb 2011 05:36
URI:	http://eprints.iisc.ac.in/id/eprint/35481

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