The C-Terminally Truncated Mtftsz-DC169 Mutant of Mycobacterium Tuberculosis Ftsz Shows Gtpase and GTP-Induced, GTP-Specific Polymerization Activities in Vitro

Gupta, Prabuddha and Anand, Syam Prasad and Srinivasan, Ramanujam and Rajeswari, Haryadi and Indi, Shantinath and Ajitkumar, Parthasarathi (2004) The C-Terminally Truncated Mtftsz-DC169 Mutant of Mycobacterium Tuberculosis Ftsz Shows Gtpase and GTP-Induced, GTP-Specific Polymerization Activities in Vitro. In: Microbiology, 150 . pp. 3906-3908.

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Abstract

We recently reported that the deletion mutant MtFtsZ-DC169 of Mycobacterium tuberculosis FtsZ (MtFtsZ), which lacked 169 C-terminal residues and retained only the first 210 residues, formed long polymers in about 30 s after the addition of GTP, like the FtsZ of Escherichia coli (EcFtsZ) (Anand et al., 2004). Both of these FtsZs were purified under denaturing conditions and refolded. By contrast, the full-length MtFtsZ, prepared under identical conditions of denaturation and refolding, formed equivalent-type long polymers in 10 min after the addition of GTP. These observations prompted us to suggest that the residues in the C-terminal region of the MtFtsZ protein might be responsible for imposing slow polymerization on MtFtsZ.

Item Type:	Journal Article
Publication:	Microbiology
Publisher:	Society for General Microbiology
Additional Information:	The copyright for this article belongs to Society for Microbiology
Department/Centre:	Division of Biological Sciences > Microbiology & Cell Biology
Date Deposited:	24 Aug 2005
Last Modified:	19 Sep 2010 04:19
URI:	http://eprints.iisc.ac.in/id/eprint/3542

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