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Development of an LH receptor assay capable of measuring serum LH/CG in a wide variety of species

Selvaraj, N and Moudgal, NR (1993) Development of an LH receptor assay capable of measuring serum LH/CG in a wide variety of species. In: Journal of Reproduction and Fertility, 98 (2). pp. 611-616.

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The development of a radioreceptor assay (RRA) that can measure serum LH in a variety of species and CG in sera and urine of pregnant women and monkeys is reported. Using sheep luteal membrane as the receptor source and I-125-labelled hLH/hCG as the tracer, dose-response (displacement) curves were obtained using hLH or hCG as standard. The addition of LH-free serum (200 mul per tube) had no affect on the standard displacement curve. The assay is simple, requires less than 90 min to complete and provides reproducible results. The sensitivity of the assay was 0.6 ng hLH per tube and the intra- and interassay variations were 9.6 and 9.8, respectively. Sera obtained from male and female bonnet monkeys (Macaca radiata) and monkey pituitary extract showed parallelism to the standard curve. The concentrations of LH measured correlated with the physiological status of the animals. Sera of rats, rabbits, hamsters, guinea-pigs, sheep and humans showed parallelism to the hLH standard curve indicating the viability of the RRA to measure serum LH of different species. Since the receptors recognize LH and CG, detection of pregnancy in monkeys and women was possible using this assay. The sensitivity of the assay for hCG was 8.7 miu per tube. This RRA could be a convenient alternative to the Leydig cell bioassay for obtaining the LH bioactivity profile of sera and biological fluids.

Item Type: Journal Article
Publication: Journal of Reproduction and Fertility
Publisher: Society for Reproduction and Fertility
Additional Information: Copyright of this article belongs to Society for Reproduction and Fertility.
Department/Centre: Division of Biological Sciences > Biochemistry
Date Deposited: 03 Feb 2011 11:58
Last Modified: 03 Feb 2011 11:58
URI: http://eprints.iisc.ac.in/id/eprint/35396

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