Katti, Muralidhar K and Chandramukhi, A (1990) Detection and characterisation of circulating cysticercal antigens in human cerebrospinal fluid. In: Serodiagnosis and Immunotherapy in Infectious Disease, 4 (6). pp. 441-450.
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With biotin labelled and unlabelled immunoglobulin fraction of anticysticercal antibodies raised in rabbits, tandem-enzyme linked immunosorbent assay (T-ELISA), capture-dot immunobinding assay (C-DIA) and reverse passive haemagglutination (RPHA) tests were developed for the detection of cysticercal antigens. The sensitivity levels were respectively, 9 ng ml−1, 2 ng ml−1 and 45 ng ml−1. All three methods were of equal specificity as none of the antigens of Mycobacterium tuberculosis, Japanese encephalitis virus and Echinococcus granulosus reacted with anticysticercal IgG. Cysticercal antigens were detected in the cerebrospinal fluid (CSF) of confirmed neurocysticercosis at sensitivity levels of 91·6% by T-ELISA, 83·33% by C-DIA and 75% by RPHA and specificity levels of >93%. Western analysis of these antigens in CSF showed mainly antigens of 64–68 kDa and 24–28 kDA. By crossed immunoelectrophoresis (CIE) with an intermediate gel technique, five circulating antigens were found to be released from scolex and fluid.
| Item Type: | Journal Article |
|---|---|
| Publication: | Serodiagnosis and Immunotherapy in Infectious Disease |
| Publisher: | Elsevier Science |
| Additional Information: | Copyright of this article belongs to Elsevier Science. |
| Keywords: | Neurocysticercosis;T-ELISA;C-DIA;RPHA;CSF and immunodiagnosis. |
| Department/Centre: | Division of Biological Sciences > Centre for Neuroscience |
| Date Deposited: | 03 Feb 2011 07:20 |
| Last Modified: | 26 Oct 2018 14:44 |
| URI: | http://eprints.iisc.ac.in/id/eprint/35200 |
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