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Detection of IgG, IgA, IgM and IgE Antibodies in Invasive Amoebiasis in Endemic Areas

Shetty, Nandini and Nagpal, Sunil and Rao, PVS and Schroder, H (1990) Detection of IgG, IgA, IgM and IgE Antibodies in Invasive Amoebiasis in Endemic Areas. In: Scandinavian Journal of Infectious Diseases, 22 (4). pp. 485-491.

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Official URL: http://informahealthcare.com/doi/abs/10.3109/00365...


Entamoeba histolytica-specific serum IgG, IgA, IgM and IgE antibodies were assayed in cases of amoebiasis in an endemic area. Patient groups consisted of amoebic liver abscess (n=18), pre-abscess hepatic amoebiasis (n=22) and amoebic colitis (n=30). Control subjects comprised 26 asymptomatic cyst passers, 13 giardiasis cases, 20 typhoid patients and 24 non-amoebic individuals. Serum IgG was assayed by ELISA, using a monoclonal anti IgG β- galactosidase (IgG β-gal) conjugate, a polyclonal avidin biotin horse radish peroxidase (AB-HRP), and a polyclonal anti IgG horse radish peroxidase (IgG HRP) conjugate. IgA and IgM were assayed by the β-gal ELISA and IgE by AB-HRP. Diagnostically significant IgG and IgA while lower IgM and IgE antibody levels were seen in extraintestinal cases. About 40% of suspected pre-abscess hepatic amoebiasis cases were confirmed by antibody estimation. All isotype levels in most dysentery cases were in the range of the controls.

Item Type: Journal Article
Publication: Scandinavian Journal of Infectious Diseases
Publisher: Informa plc
Additional Information: Copyright of this article belongs to Informa plc.
Department/Centre: Division of Biological Sciences > Biochemistry
Date Deposited: 03 Feb 2011 08:59
Last Modified: 03 Feb 2011 08:59
URI: http://eprints.iisc.ac.in/id/eprint/35196

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