Detection of a CpA methylase in an insect system: characterization and substrate specificity

Devajyothi, Chalasani and Brahmachari, Vani (1992) Detection of a CpA methylase in an insect system: characterization and substrate specificity. In: Molecular and Cellular Biochemistry, 110 (2). pp. 103-111.

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Abstract

A cytosine-specific DNA methyltransferase (EC 2.1.1.37) has been purified to near homogeneity from a mealybug (Planococcus lilacinus). The enzyme can methylate cytosine residues in CpG sequences as well as CpA sequences. The apparent molecular weight of the enzyme was estimated as 135,000 daltons by FPLC. The enzyme exhibits a processive mode of action and a salt dependance similar to mammalian methylases. Mealybug methylase exhibits a preference for denatured DNA substrates.

Item Type:	Journal Article
Publication:	Molecular and Cellular Biochemistry
Publisher:	Springer
Additional Information:	Copyright of this article belongs to Springer.
Keywords:	DNA methylation;coccids;processive enzym;RNA inhibition;CpI methylation.
Department/Centre:	Division of Biological Sciences > Microbiology & Cell Biology
Date Deposited:	25 Jan 2011 04:51
Last Modified:	25 Jan 2011 04:51
URI:	http://eprints.iisc.ac.in/id/eprint/35166

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