Antigenic determinants on chicken riboflavin carrier protein. A study with monoclonal antibodies

Velu, Kuzhandhai N and Karande, Anjali A and Adiga, PR (1992) Antigenic determinants on chicken riboflavin carrier protein. A study with monoclonal antibodies. In: Journal of Biosciences, 17 (2). pp. 151-165.

PDF Antigenic_determinants.pdf - Published Version Restricted to Registered users only Download (516kB) | Request a copy

Abstract

Monoclonal antibodies raised against chicken egg white riboflavin carrier protein were classified into seven categories each recognizing a distinct epitope. Of these, six were directed against conformation dependent epitopes and one to a sequential epitope. The roles of lysine residues and the post-translationally attached phosphate and oligosaccharide moieties in the antigenicity of riboflavin carrier protein recognized by the monoclonal antibodies were investigated. The binding region of three monoclonal antibodies could be located within the 87–219 amino acid sequence of the protein and one antibody among these recognized a sequence of 182–204 amino acid residues. All the monoclonal antibodies were able to recognize riboflavin carrier proteins present in the sera of pregnant rats, cows and humans indicating that the epitopes to which they are directed are conserved through evolution from chicken to the human.

Item Type:	Journal Article
Publication:	Journal of Biosciences
Publisher:	Indian Academy of Sciences
Additional Information:	Copyright of this article belongs to Indian Academy of Sciences.
Keywords:	Riboflavin carrier protein;antigenic determinants;monoclonal antibodies.
Department/Centre:	Division of Biological Sciences > Biochemistry
Date Deposited:	28 Jan 2011 05:12
Last Modified:	28 Jan 2011 05:12
URI:	http://eprints.iisc.ac.in/id/eprint/35080

Actions (login required)

View Item