New pathway for the biodegradation of indole in Aspergillus niger

Kamath, AV and Vaidyanathan, CS (1990) New pathway for the biodegradation of indole in Aspergillus niger. In: Applied and Environmental Microbiology, 56 (1). pp. 275-280.

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Abstract

Indole and its derivatives form a class of toxic recalcitrant environmental pollutants. The growth of Aspergillus niger was inhibited by very low concentrations (0.005 to 0.02%) of indole, even when 125- to 500-fold excess glucose was present in the medium. When 0.02% indole was added, the fungus showed a lag phase for about 30 h and the uptake of glucose was inhibited. Indole was metabolized by a new pathway via indoxyl (3-hydroxyindole), N-formylanthranilic acid, anthranilic acid,2,3-dihydroxybenzoic acid, and catechol, which was further degraded by ortho cleavage. The enzymes N-formylanthranilate deformylase, anthranilate hydroxylase, 2,3-dihydroxybenzoate decarboxylase, and catechol dioxygenase were induced by indole as early as after 5 h of growth, and their activities were demonstrated in a cell-free system.

Item Type:	Journal Article
Publication:	Applied and Environmental Microbiology
Publisher:	American Society for Microbiology
Additional Information:	Copyright of this article belongs to American Society for Microbiology.
Department/Centre:	Division of Biological Sciences > Biochemistry
Date Deposited:	27 Apr 2011 05:10
Last Modified:	27 Apr 2011 05:10
URI:	http://eprints.iisc.ac.in/id/eprint/35028

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