Isolation and characterization of thiamin-binding protein from chicken egg white

Muniyappa, K and Adiga, PR (1979) Isolation and characterization of thiamin-binding protein from chicken egg white. In: Biochemical Journal, 177 (3). pp. 887-894.

PDF Isolation.pdf - Published Version Restricted to Registered users only Download (1MB) | Request a copy

Abstract

A thiamin-binding protein was isolated and characterized from chicken egg white by affinity chromatography on thiamin pyrophosphate coupled to aminoethyl-Sepharose. The high specificity of interaction between the thiamin-binding protein and the riboflavin-binding protein of the egg white, with a protein/protein molar ratio of 1.0, led to the development of an alternative procedure that used the riboflavin-binding protein immobilized on CNBr-activated Sepharose as the affinity matrix. The thiamin-binding protein thus isolated was homogeneous by the criteria of polyacrylamide-gel disc electrophoresis, double immunodiffusion and sodium dodecyl sulphate/polyacrylamide-gel electrophoresis, had a mol.wt. of 38,000 +/- 2000 and was not a glycoprotein. The protein bound [14C]thiamin was a molar ratio of 1.0, with dissociation constant (Kd) 0.3 micrometer.

Item Type:	Journal Article
Publication:	Biochemical Journal
Publisher:	Portland Press
Additional Information:	Copyright of this article belongs to Portland Press.
Department/Centre:	Division of Biological Sciences > Biochemistry
Date Deposited:	23 Dec 2010 07:45
Last Modified:	01 Mar 2012 06:40
URI:	http://eprints.iisc.ac.in/id/eprint/34361

Actions (login required)

View Item