Nucleosome core histone complex isolated gently and rapidly in 2 M NaCl is octameric

Philip, M and Jamaluddin, M and Sastry, RV and Chandra, HS (1979) Nucleosome core histone complex isolated gently and rapidly in 2 M NaCl is octameric. In: PNAS, 76 (10). pp. 5178-5182.

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Abstract

A relatively stable specific complex of the chromatin core histones H2A, H2B, H3, and H4 has been obtained in 2 M NaCl/25 mM sodium phosphate buffer, pH 7.0. The histone core complex has an apparent specific volume of 0.73 ml/g. Its sedimentation coefficient was dependent on rotor speed (angular velocity, omega) and attained different stable values at low and high rotor speeds. The drop in sedimentation coefficient occurred sharply between omega 2 values of about 9 x 10(6) and 1.1 x 10(7) (radians/sec)2. The s020,w corresponding to zero angular velocity (1 atmosphere pressure) was 6.6 S +/- (SEM) 0.1 S. At high rotor speeds the value decreased to 3.8 S +/- 0.1 S. The core complex has a diffusion coefficient, D20,w, of 5.4 x 10(-7) cm2/sec and a molecular weight of 108,000 +/- (SD) 2500.

Item Type:	Journal Article
Publication:	PNAS
Publisher:	National Academy of Sciences
Additional Information:	Copyright of this article belongs to National Academy of Sciences.
Department/Centre:	Division of Biological Sciences > Microbiology & Cell Biology Division of Physical & Mathematical Sciences > Centre for Theoretical Studies (Ceased to exist at the end of 2003)
Date Deposited:	27 Dec 2010 06:19
Last Modified:	21 Oct 2011 09:23
URI:	http://eprints.iisc.ac.in/id/eprint/34241

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