Rao, US and Murthy, SK (1991) The effects of beta-mercaptoethanol and sodium dodecyl sulfate on the Humicola insolens beta-glucosidase. In: Biochemistry International, 23 (2). 343 -348.
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Hydrolysis of p-nitrophenyl-beta-D-glucoside by the beta-glucosidase of a thermophilic and cellulolytic fungus, Humicola insolens was stimulated by two-fold in the presence of high concentrations of beta-mercaptoethanol. This enzyme did not have any free sulfhydryl groups and high concentrations of beta-mercaptoethanol (5% v/v) reduced all of the three disulfide bonds present in the enzyme. In contrast, the hydrolysis of cellobiose and cellulose polymers was inhibited by 50% under the same conditions. Sodium dodecyl sulfate (1% w/v) even in combination with beta-mercaptoethanol did not show any significant effects on this enzyme. These unusual properties suggest that this enzyme may be of significant importance for understanding the structure of the enzyme.
| Item Type: | Journal Article |
|---|---|
| Publication: | Biochemistry International |
| Publisher: | Academic Press Aust |
| Additional Information: | Copyright of this article belongs to Academic Press Aust. |
| Keywords: | Thermoascus-Aurantiacus;Thermophilic Fungus;Purification; Endocellulase. |
| Department/Centre: | Division of Biological Sciences > Biochemistry |
| Date Deposited: | 07 Dec 2010 09:43 |
| Last Modified: | 07 Dec 2010 09:43 |
| URI: | http://eprints.iisc.ac.in/id/eprint/34236 |
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