Moorthy, B and Madyastha, P and Madyastha, KM (1991) Destruction Of Rat-Liver Microsomal Cytochrome-P-450 Invitro By A Monoterpene Ketone, Pulegone A Hepatotoxin. In: Indian Journal of Chemistry - Section B: Organic and Medicinal Chemistry, 30 (2). pp. 138-146.
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Significant destruction (68%) of liver microsomal cytochrome P-450 and homogeneous cytochrome P-450 purified from PB-treated rats is noticed upon incubation with 10 mM pulegone at 37-degrees-C for 30 min. There is also a concomitant loss of heme. The destructive phenomenon does not require metabolic activation of pulegone. The destruction of purified cytochrome P-450 is time-dependent and saturable. Structure-activity studies suggest that an alpha-isopropylidine ketone unit with a methyl positioned para to the isopropylidine group as in pulegone is necessary for the in vitro destruction of cytochrome P-450. SKF-525A at a concentration of 4-mM obliterates the destruction of cytochrome P-450 by pulegone. Experiments with C-14-pulegone suggest that pulegone or its rearranged product binds covalently to the prosthetic heme of cytochrome P-450.
| Item Type: | Journal Article |
|---|---|
| Publication: | Indian Journal of Chemistry - Section B: Organic and Medicinal Chemistry |
| Publisher: | National Institute of Science Communication and Information Resources |
| Additional Information: | Copyright of this article belongs to National Institute of Science Communication and Information Resources. |
| Keywords: | Metabolism;Enzymes;Invivo;Heme;Inactivation;Reductase. |
| Department/Centre: | Division of Chemical Sciences > Organic Chemistry |
| Date Deposited: | 10 Nov 2010 07:30 |
| Last Modified: | 10 Nov 2010 07:30 |
| URI: | http://eprints.iisc.ac.in/id/eprint/33733 |
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