Hemin-mediated oxidation of dithiothreitol reduces oxygen to H2O

Devi , Usha S and Ramasarma, T (1987) Hemin-mediated oxidation of dithiothreitol reduces oxygen to H2O. In: Molecular and Cellular Biochemistry, 77 (2). pp. 111-120.

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Abstract

Hemin catalyses the oxidation of dithiothreitol. One mole of oxygen is consumed for every 2 moles of dithiothreitol oxidized and the product is shown by spectral studies to be the intramolecular disulphide. The reaction shows a specificity for dithiol and for free heme moieties. Hemin molecules exhibit cooperativity in oxygen reduction. Oxygen radicals do not seem to be involved. H2O2 is not required for this oxidation of dithiothreitol and does not appear to be an intermediate in the reduction of O2 to H2O. However, an independent minor reaction involving a 2-electron transfer with the formation of H2O2 also occurs. These studies on the hemin-catalyzed oxidation of dithiothreitol provide a chemical model for a direct 4-electron reduction of O2 to H2O.

Item Type:	Journal Article
Publication:	Molecular and Cellular Biochemistry
Publisher:	Springer
Additional Information:	Copyright of this article belongs to Springer.
Keywords:	Hemin catalysis;DTT oxidation;oxygen reduction.
Department/Centre:	Division of Biological Sciences > Biochemistry
Date Deposited:	29 Oct 2010 06:34
Last Modified:	29 Oct 2010 06:34
URI:	http://eprints.iisc.ac.in/id/eprint/33392

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