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Effect of isonicotinic acid hydrazide-copper complex on Rous sarcoma virus and its genome RNA

Antony, A and Ramakrishnan, T and Mikelens, Peter and Jackson, Jean and Levinson, Warren (1978) Effect of isonicotinic acid hydrazide-copper complex on Rous sarcoma virus and its genome RNA. In: Bioinorganic Chemistry, 9 (1). 23-34 .

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Official URL: http://dx.doi.org/10.1016/S0006-3061(00)82003-7


The copper complex of the antituberculous drug, isonicotinic acid hydrazide (INH), inhibits the RNA-dependent DNA polymerase of Rous sarcoma virus and inactivates its ability to malignantly transform chick embryo cells. The INH-copper complex binds to the 70S genome RNA of Rous sarcoma virus (RSV), which may account for its ability to inhibit the RNA-dependent DNA polymerase. The complex binds RNA more effectively than DNA in contrast to M-IBT-copper complexes, which bind both types of nucleic acids equally. The homopolymers, poly rA and poly rU, are bound by the INH-copper complex to a greater extent than poly rC. Isonicotinic acid hydrazide alone and CuSO4 alone bind neither DNA, RNA, poly (rA), poly (rU), nor poly (rC). However, CuSO4 alone binds poly (rI); INH alone does not. In addition to viral DNA synthesis, chick-embryo cell DNA synthesis is inhibited by the INH-copper complex. The extent of inhibition of cellular DNA synthesis is greater than that of cellular RNA and protein synthesis. No selective inhibition of transformation in cells previously infected with Rous sarcoma virus is observed.

Item Type: Journal Article
Publication: Bioinorganic Chemistry
Publisher: Elsevier Science
Additional Information: Copyright of this article belongs to Elsevier Science.
Department/Centre: Division of Biological Sciences > Microbiology & Cell Biology
Date Deposited: 27 Oct 2010 06:57
Last Modified: 13 Feb 2019 09:11
URI: http://eprints.iisc.ac.in/id/eprint/33184

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