Chemical approaches of penicillin allergy—III Isolation of the penicillin-free carrier receptor protein (CRP) on a polymeric 7-deoxy penicillin analogue template and its role in penicillin immunogeneses in rabbit and man

Nataraj, CV and Mandal, Chitra and Bhattacharyya, PK (1978) Chemical approaches of penicillin allergy—III Isolation of the penicillin-free carrier receptor protein (CRP) on a polymeric 7-deoxy penicillin analogue template and its role in penicillin immunogeneses in rabbit and man. In: Proceedings of the Indian Academy of Sciences Section -A, 87 (1). pp. 1-12.

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Abstract

Specific penicillin-carrier receptor proteins (CRP) have been isolated from the sera of penicillin allergic rabbits and human subjects in the unconjugated native state in electrophoretically homogeneous form by employing a synthetic polymeric affinity template containing the 7-deoxy analogue of penicillin G. The synthesis of the 7-deoxy analogue has been described. In this affinity system the antipenicillin-antibody is desorbed by 0·9M thiourea and the CRP in 8M urea. The CRP after incubation with penicillin is converted into the full-fledged antigen. Studies on the origin of CRP and the nature of antibody as well as comparative studies on the properties of the rabbit antibody and those of antibodies elicited by a BSA-BPO conjugate are reported.

Item Type:	Journal Article
Publication:	Proceedings of the Indian Academy of Sciences Section -A
Publisher:	Indian Academy of Sciences
Additional Information:	Copyright of this article belongs to Indian Academy of Sciences.
Keywords:	Penicillin allergy -immunology -receptor -affinity chromatology -antigen-antibody interaction - hapten.
Department/Centre:	Division of Chemical Sciences > Organic Chemistry
Date Deposited:	06 Oct 2010 09:42
Last Modified:	06 Oct 2010 09:42
URI:	http://eprints.iisc.ac.in/id/eprint/32992

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