Manjunath, GS and Dufresne, MJ (1988) Plasmid DNA mediated transfer of the herpes simplex virus thymidine kinase gene to a new bromodeoxyuridine resistant variant of human primary lung carcinoma cells. In: Biochemistry International, 16 (1). pp. 149-156.
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A human primary lung carcinoma cell line (HPL-R1) established from the tumor biopsy of a lung cancer patient, lacking in cytochrome P1-450 [aryl hydrocarbon (benzo[a]pyrene) hydroxylase (AHH)], was cloned and used to obtain variants deficient in the expression of thymidine-kinase via treatment with 5-bromo-2'-deoxyuridine, and selection for drug resistance phenotype. The variant cell line, precharacterized for thymidine kinase negative phenotype, was transfected with the thymidine kinase gene bearing p R-tk and px1-tk plasmids. Transfections from both the plasmids, demonstrated a frequency of 5.5 X 10(-5). The transfectants showed a 76-100% retention of the transferred phenotype. These data suggest that transfection in variant human cells can approach significant levels of stability observed with rodent cell recipients.
| Item Type: | Journal Article |
|---|---|
| Publication: | Biochemistry International |
| Publisher: | Academic Press Aust |
| Additional Information: | Copyright of this article belongs to Academic Press Aust. |
| Department/Centre: | Division of Biological Sciences > Biochemistry |
| Date Deposited: | 29 Sep 2010 10:09 |
| Last Modified: | 29 Sep 2010 10:09 |
| URI: | http://eprints.iisc.ac.in/id/eprint/32499 |
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