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Oxidative activities in mitochondria-like particles from Setaria digitata, a filarial parasite

Raj, Kaleysa R and Puranam, RS and Kurup, Ramakrishna CK and Ramasarma, T (1988) Oxidative activities in mitochondria-like particles from Setaria digitata, a filarial parasite. In: Biochemical Journal, 256 (2). pp. 559-564.

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The oxidative metabolic potential of Setaria digitata, a filarial parasite found in the intraperitoneal cavity of cattle, was investigated. These worms showed active wriggling movements which were not affected by respiratory poisons such as cyanide, rotenone and malonate. They also possessed cyanide-insensitive and glucose-independent oxygen consumption pathways. By differential centrifugation of sucrose homogenates, a fraction containing mitochondria-like particles was obtained in which the activity of the marker enzyme, succinate dehydrogenase, was recovered. This fraction catalysed succinate- and NADH-dependent reduction of both cytochrome c and dyes. Oxygen uptake found with succinate, NADH and ascorbate as substrates was not sensitive to cyanide. Cytochromes could not be detected in either this fraction or homogenates of the worms. H2O2 generation with a number of substrates and lipid peroxidation by measuring malondialdehyde formed as well as by accompanying oxygen uptake were demonstrated in the mitochondria-like particles. A lipid quinone, possibly with a short side chain and related to ubiquinone, was detected in the worms. The results suggested the existence of two cyanide-insensitive oxygen-consuming reactions in Setaria: one respiratory substrate-independent lipid peroxidation, and a second substrate-dependent reaction that requires an auto-oxidizable quinone but not a cytochrome system.

Item Type: Journal Article
Publication: Biochemical Journal
Publisher: Portland Press
Additional Information: Copyright of this article belongs to Portland Press.
Department/Centre: Division of Biological Sciences > Biochemistry
Date Deposited: 24 Sep 2010 10:09
Last Modified: 24 Sep 2010 10:09
URI: http://eprints.iisc.ac.in/id/eprint/32442

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