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In vitro meristem cloning of Eucalyptus tereticornis Sm

Rao, Sankara K (1988) In vitro meristem cloning of Eucalyptus tereticornis Sm. In: Plant Cell Reports, 7 (7). 546 -549.

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Rapid multiplication of axillary meristems and direct shoot development occurred from nodal explants of mature Eucalyptus tereticornis Sm. with 5.3 mgrM NAA, 1.1 mgrM IAA and 4.4 mgrM BA in Murashige-Skoog medium. Repeated subcultures of the second generation shoot cultures into low cytokinin-auxin containing media (0.44�0.88 mgrM BA+0.1 mgrM NAA) yielded axillary microshoots in large numbers. Half-strength MS liquid medium with 4.9 mgrM IBA, 5.5 mgrM IAA and 5.3 mgrM NAA for four days, half-strength semi-solid hormonefree MS medium with charcoal, and MS liquid medium without charcoal and hormones, in sequence, induced rooting of shoots in the dark. This system is suitable for the mass propagation of this difficult-to-root eucalypt.

Item Type: Journal Article
Publication: Plant Cell Reports
Publisher: Springer
Additional Information: Copyright of this article belongs to Springer.
Department/Centre: Division of Biological Sciences > Biochemistry
Date Deposited: 22 Sep 2010 09:46
Last Modified: 22 Sep 2010 09:46
URI: http://eprints.iisc.ac.in/id/eprint/32339

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