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Interaction of rat testis protein, TP, with nucleosome core particle

Singh, J and Rao, MRS (1988) Interaction of rat testis protein, TP, with nucleosome core particle. In: Biochemistry International, 17 (4). pp. 701-710.

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Official URL: http://www.ncbi.nlm.nih.gov/pubmed/3240317


Circular dichroism studies have revealed that addition of testis specific protein, TP in vitro, to rat testes nucleosome core particle resulted in a decrease in the compaction of the core particle DNA. This was also corroborated by thermal denaturation analysis. Addition of TP to nucleosome core particle resulted in the conversion of a biphasic transition towards a single phase. However, at the same time there was a 20% reduction in the overall hyperchromicity of core particle DNA at core particle to TP molar ratios of 1:2 and 1:3. These observations along with our earlier report, showing the DNA melting properties of TP, suggest that TP may play an important role in the disassembly process of nucleosome core particle during spermiogenesis.

Item Type: Journal Article
Publication: Biochemistry International
Publisher: Academic Press Aust
Additional Information: Copyright of this article belongs to Academic Press Aust.
Department/Centre: Division of Biological Sciences > Biochemistry
Date Deposited: 22 Sep 2010 05:52
Last Modified: 22 Sep 2010 05:52
URI: http://eprints.iisc.ac.in/id/eprint/32318

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