Deobagkar , DN and Gopinathan, KP (1978) Studies on transfer RNA from mycobacteria. In: Canadian Journal of Microbiology / Revue canadienne de microbiologie, 24 (6). 693-702 .
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Active preparations of tRNA and aminoacyl-tRNA synthetases have been isolated from exponentially growing cells of Mycobacterium smegmatis and Mycobacterium tuberculosis H37Rv. Though the aminoacyl-tRNA synthetases of older cells retain their activity, the tRNAs seem to undergo modification and show poorer activity. The mycobacterial enzyme preparations catalyse homologous and heterologous aminoacylation between tRNA from the two species (M. smegmatis and M. tuberculosis H37Rv) or from Escherichia coli, with equal efficiency; tRNA samples from eukaryotic cells (yeast and rat liver) do not serve as substrates for the mycobacterial synthetases. The analytical separation of the different amino acid specific tRNAs from M. smegmatis resembles the pattern found in other bacteria. Purification of valine- (three species) and methionine-specific tRNA (two species) to 70-80% purity has been accomplished by using column-chromatographic techniques. Of the two species of tRNAMet, one can be formylated in the presence of formyl tetrahydrofolate and the transformylase from mycobacteria.
| Item Type: | Journal Article |
|---|---|
| Publication: | Canadian Journal of Microbiology / Revue canadienne de microbiologie |
| Publisher: | NRC Research Press |
| Additional Information: | Copyright of this article belongs to NRC Research Press. |
| Department/Centre: | Division of Biological Sciences > Microbiology & Cell Biology |
| Date Deposited: | 21 Sep 2010 07:14 |
| Last Modified: | 21 Sep 2010 07:14 |
| URI: | http://eprints.iisc.ac.in/id/eprint/32300 |
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