Subrahmanyam, YVBK and Padayatty, JD (1988) Identification and expression of the 20 kd structural protein gene of colitis bacteriophage. In: Biochemistry International, 16 (4). pp. 701-712.
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The 2.3 kb BamHI fragment from the colitis bacteriophage DNA was transcribed and translated into a 20 kd structural protein P6, in a coupled transcription-translation system derived from Escherichia coli. This protein was expressed in vivo by the 2.3 kb DNA cloned in pBR322. The gene with the regulatory elements for this protein was located on the 680 bp AvaII fragment of the insert DNA. It hybridized with two RNAs of sizes 520 and 1630 nucleotides indicating that both are messengers for the 20 kd protein. Dot-blot hybridization showed that the transcripts for P6 reached a maximum level at 12 min after phage infection.
| Item Type: | Journal Article |
|---|---|
| Publication: | Biochemistry International |
| Publisher: | Academic Press Aust |
| Additional Information: | Copyright of this article belongs to Academic Press Aust. |
| Department/Centre: | Division of Biological Sciences > Biochemistry |
| Date Deposited: | 21 Sep 2010 09:33 |
| Last Modified: | 21 Sep 2010 09:33 |
| URI: | http://eprints.iisc.ac.in/id/eprint/32278 |
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