Expression of drug resistance markers by spheroplasts ofMycobacterium smegmatis after fusion

Sadhu, Chanchal and Gopinathan, KP (1988) Expression of drug resistance markers by spheroplasts ofMycobacterium smegmatis after fusion. In: Journal of Genetics, 67 (2). pp. 67-73.

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Abstract

Mycobacterial spheroplasts were prepared by treatment of the glycinesensitized cells with a combination of lipase and lysozyme. They were stable for several hours at room temperature but were lysed on treatment with 0.1% sodium dodecyl sulfate. The spheroplasts could be regenerated on a suitable medium. Fusion and regeneration of the spheroplasts were attempted using drug resistant mutant strains ofM. smegmalis. Recombinants were obtained from spheroplast fusion mediated by polyethylene glycol and dimethyl sulfoxide. Simultaneous expression of rccombinant properties was observed only after an initial lag in the isolated clones. This has been explained as due to “chromosome inactivation” in the fused product.

Item Type:	Journal Article
Publication:	Journal of Genetics
Publisher:	Indian Academy of Sciences
Additional Information:	Copyright of this article belongs to Indian Academy of Sciences.
Keywords:	Spheroplast fusion;mycobacteria;prophage activation; recombination;chronaosonae inactivation.
Department/Centre:	Division of Biological Sciences > Microbiology & Cell Biology
Date Deposited:	17 Sep 2010 05:39
Last Modified:	19 Sep 2010 06:16
URI:	http://eprints.iisc.ac.in/id/eprint/32187

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