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Binding of deoxyadenylate and deoxycytidylate antibodies to double-stranded DNA

Vaishnav, YN and Antony, A (1988) Binding of deoxyadenylate and deoxycytidylate antibodies to double-stranded DNA. In: Biochemical and Biophysical Research Communications, 154 (1). pp. 118-123.

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Official URL: http://dx.doi.org/10.1016/0006-291X(88)90658-4


Antibodies raised against deoxyadenylate and deoxycytidylate were found to react with double stranded DNA as assessed by highly sensitive avidin-biotin microELISA. The binding was specific as it was completely inhibited by the homologous hapten. The antibodies did not react with tRNA and rRNA. These antibodies were also shown to react with supercoiled and relaxed forms of pBR322 DNA as demonstrated by gel retardation assay. ssDNA, single-stranded DNA; dsDNA, double-stranded DNA; CT DNA, calf thymus DNA; AB microELISA, avidin-biotin microELISA; dpA, deoxyadenylate; dpC, deoxycytidylate; avidin-HRP, avidin-horseradish peroxidase

Item Type: Journal Article
Publication: Biochemical and Biophysical Research Communications
Publisher: Elsevier science
Additional Information: Copyright of this article belongs to Elsevier science.
Department/Centre: Division of Biological Sciences > Microbiology & Cell Biology
Date Deposited: 06 Sep 2010 09:54
Last Modified: 19 Sep 2010 06:16
URI: http://eprints.iisc.ac.in/id/eprint/32050

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