Role of a PAS sensor domain in the Mycobacterium tuberculosis transcription regulator Rv1364c

Jaiswal, Ravi Kumar and Manjeera, G and Gopal, B (2010) Role of a PAS sensor domain in the Mycobacterium tuberculosis transcription regulator Rv1364c. In: Biochemical and Biophysical Research Communications, 398 (3). pp. 342-349.

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Abstract

The Mycobacterium tuberculosis transcriptional regulator Rv1364c regulates the activity of the stress response sigma factor sigma(F). This multi-domain protein has several components: a signaling PAS domain and an effector segment comprising of a phosphatase, a kinase and an anti-anti-sigma factor domain. Based on Small Angle X-ray Scattering (SAXS) data, Rv1364c was recently shown to be a homo-dimer and adopt an elongated conformation in solution. The PAS domain could not be modeled into the structural envelope due to poor sequence similarity with known PAS proteins. The crystal structure of the PAS domain described here provides a structural basis for the dimerization of Rv1364c. It thus appears likely that the PAS domain regulates the anti-sigma activity of Rv1364c by oligomerization. A structural comparison with other characterized PAS domains reveal several sequence and conformational features that could facilitate ligand binding - a feature which suggests that the function of Rv1364c could potentially be governed by specific cellular signals or metabolic cues. (C) 2010 Elsevier Inc. All rights reserved.

Item Type:	Journal Article
Publication:	Biochemical and Biophysical Research Communications
Publisher:	Elsevier Science
Additional Information:	Copyright of this article belongs to Elsevier Science.
Keywords:	PAS;Environmental sensor;Transcriptional regulator; Dimerization; Rv1364c
Department/Centre:	Division of Biological Sciences > Molecular Biophysics Unit
Date Deposited:	03 Sep 2010 11:13
Last Modified:	19 Sep 2010 06:15
URI:	http://eprints.iisc.ac.in/id/eprint/32001

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