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Sequence specificity in spermine-induced structural changes in CG-oligomers

Majumder, K and Brahmachari, SK (1989) Sequence specificity in spermine-induced structural changes in CG-oligomers. In: Biochemistry International, 18 (2). pp. 455-465.

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Official URL: http://www.ncbi.nlm.nih.gov/pubmed/2764957


The role of spermine in inducing A-DNA conformation in deoxyoligonucleotides has been studied using CCGG and GGCC as model sequences. It has been found that while CCGG adopts an alternating B-DNA conformation in low salt solution at low temperature, addition of spermine to this medium induces a B --greater than A transition. In contrast, the A-DNA-like structure of GGCC in low salt solution at low temperature does not change under the influence of spermine. This suggests a sequence-dependent behaviour of spermine. Further these results suggest that the A-DNA conformation observed in the crystals of d(iCCGG) and d(GGCC)2 might have been due to the presence of spermine in the crystallization cocktail.

Item Type: Journal Article
Publication: Biochemistry International
Publisher: Academic Press Aust
Additional Information: Copyright of this article belongs to Academic Press Aust.
Department/Centre: Division of Biological Sciences > Molecular Biophysics Unit
Date Deposited: 26 Aug 2010 09:08
Last Modified: 26 Aug 2010 09:08
URI: http://eprints.iisc.ac.in/id/eprint/31543

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