Kumar, Harish PM and Virupaksha, Tumkur K and Vithayathil, Paul Joseph (1978) Sorghum proteinase inhibitors: purification and some biochemical properties. In: International Journal of Peptide & Protein Research, 12 (4). pp. 185-196.
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An investigation has been carried out on the proteinase inhibitors of grain sorghum (Sorghum bicolor (L.) Moench). One of the inhibitors has been isolated in a pure form and characterized. The proteinase inhibitor was extracted from the acetone-defatted sorghum meal and purified by selective thermal denaturation, ammonium sulfate fractionation, Sephadex gel filtration and DEAE-cellulose chromatography (DEAE-preparation II). This preparation was demonstrated to be a mixture of three inhibitor components by polyacrylamide disc gel electrophoresis. Further resolution of this mixture into Inhibitors I to III was achieved by QAE-Sephadex chromatography. Sorghum Inhibitor III was homogeneous by the criteria of disc gel electrophoresis and has been more fully characterized. A molecular weight of 25,000 was obtained for Inhibitor III by gel filtration and was in agreement with the value calculated from the amino acid composition of the inhibitor. The N-terminal amino acid residue of Inhibitor III, a single chain protein, was isoleucine. Sorghum proteinase inhibitors inhibit specifically the serine proteinases and are inactive towards the other classes of proteinases. Inhibitor III is primarily a chymotrypsin inhibitor, whereas Inhibitors I and II inhibit both trypsin and chymotrypsin.
| Item Type: | Journal Article |
|---|---|
| Publication: | International Journal of Peptide & Protein Research |
| Publisher: | Munksgaard int publ ltd |
| Additional Information: | Copyright of this article belongs to Munksgaard int publ ltd. |
| Keywords: | Isolation of sorghum isoinhibitors;plant Proteinasenhibitors;proteinase inhibitors;sorghum bicolor (L) moench;sorghum proteinase inhibitors;specificity of sorghum inhibitors. |
| Department/Centre: | Division of Biological Sciences > Biochemistry |
| Date Deposited: | 23 Aug 2010 05:32 |
| Last Modified: | 23 Aug 2010 05:32 |
| URI: | http://eprints.iisc.ac.in/id/eprint/31471 |
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