Hepatotoxicity of pulegone in rats: Its effects on microsomal enzymes, in vivo

Moorthy, Bhagavatula and Madyastha, Prema and Madyastha, Madhava K (1989) Hepatotoxicity of pulegone in rats: Its effects on microsomal enzymes, in vivo. In: Toxicology, 55 (3). pp. 327-337.

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Abstract

Oral administration of pulegone (400 mg/kg) to rats once daily for five days caused significant decreases in the levels of liver microsomal cytochrome P-450 and heme. Cytochrome b5 and NAD(P)H-cytochrome c-reductase activities were not affected. Massive hepatotoxicy accompanied by an increase in serum glutamate pyruvate transaminase (SGPT) and a decrease in glucose-6-phosphatase were observed upon treatment with pulegone. A significant decrease in aminopyrine N-demethylase was also noticed after pulegone administration. Menthone or carvone (600 mg/kg), compounds related to pulegone, when administered orally did not cause any decrease in cytochrome P-450 levels. The hepatotoxic effects of pulegone were both dose and time dependent. Pretreatment of rats with phenobarbital (PB) or diethylmaleate (DEM) potentiated the hepatotoxicity caused by pulegone, whereas, pretreatment with 3-methylcholanthrene (3-MC) or piperonyl butoxide protected from it. It appears that a PB induced cytochrome P-450 catalysed reactive metabolite(s) may be responsible for the hepatotoxicity caused by pulegone.

Item Type:	Journal Article
Publication:	Toxicology
Publisher:	Elsevier Science
Additional Information:	Copyright of this article belongs to Elsevier Science.
Keywords:	Pulegone;Cytochrome P-450;Hepatotoxicity;Destruction;Drug Metabolizing enzymes;Glutamate pyruvate transminase.
Department/Centre:	Division of Chemical Sciences > Organic Chemistry
Date Deposited:	19 Aug 2010 13:06
Last Modified:	19 Sep 2010 06:14
URI:	http://eprints.iisc.ac.in/id/eprint/31426

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