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Direct somatic embryogenesis in zygotic embryos of nutmeg (Myristica fragrans Houtt.)

Iyer, RI and Jayaraman, G and Gopinath, PM and Sita, Lakshmi G (2000) Direct somatic embryogenesis in zygotic embryos of nutmeg (Myristica fragrans Houtt.). In: Tropical Agriculture, 77 (2). pp. 98-105.

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Direct somatic embryogenesis from isolated intact as well as broken zygotic embryos and in vitro plantlets of nutmeg (Myristica fragrans Houtt.) was obtained. Enhanced embryogenic response was associated with broken zygotic embryos. Activated charcoal and light were the critical factors for induction of somatic embryogenesis in nutmeg. Histological evaluation revealed the presence of globular and cotyledonary stages. The somatic embryos underwent partial germination after a six-month lag period. A wide range of abnormal embryos were observed. The somatic embryos synthesised chlorophyll, exhibited phenylalanine ammonia lyase activity, synthesised phenolics, and could serve as a stable source of secondary metabolites of nutmeg which are commercially important.

Item Type: Journal Article
Publication: Tropical Agriculture
Publisher: Tropical Agriculture
Additional Information: Copyright of this article belongs to Tropical Agriculture.
Department/Centre: Division of Biological Sciences > Microbiology & Cell Biology
Date Deposited: 18 Aug 2010 11:17
Last Modified: 18 Aug 2010 11:17
URI: http://eprints.iisc.ac.in/id/eprint/31413

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