New evidences on Tau-DNA interactions and relevance to neurodegeneration

Padmaraju, Vasudevaraju and Indi, Shantinath Satappa and Rao, Kosagi Sharaf Jagannatha (2010) New evidences on Tau-DNA interactions and relevance to neurodegeneration. In: Neurochemistry International, 57 (1). pp. 51-57.

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Abstract

Tau is mainly distributed in cytoplasm and also found to be localized in the nucleus. There is limited data on DNA binding potential of Tau.We provide novel evidence on nicking of DNA by Tau. Tau nicks the supercoiled DNA leading to open circular and linear forms. The metal ion magnesium (a co-factor for endonuclease) enhanced the Tau DNA nicking ability, while an endonuclease specific inhibitor,aurinetricarboxylic acid (ATA) inhibited the Tau DNA nicking ability Further, we also evidenced that Tau induces B-C-A mixed conformational transition in DNA and also changes DNA stability. Tau-scDNA complex is more sensitive to DNAse I digestion indicating stability changes in DNA caused by Tau. These findings indicate that Tau alters DNA helicity and integrity and also nicks the DNA. The relevance of these novel intriguing findings regarding the role Tau in neuronal dysfunction is discussed. (C) 2010 Elsevier Ltd. All rights reserved.

Item Type:	Journal Article
Publication:	Neurochemistry International
Publisher:	Elsevier Science
Additional Information:	Copyright of this article belongs to Elsevier Science.
Keywords:	Tau;DNA conformation DNA nicking;Aurinetricarboxylic acid; Neurodegeneration; Alzheimer's disease.
Department/Centre:	Division of Biological Sciences > Microbiology & Cell Biology
Date Deposited:	09 Aug 2010 12:47
Last Modified:	19 Sep 2010 06:13
URI:	http://eprints.iisc.ac.in/id/eprint/31030

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