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A novel triacylglycerol biosynthesis in the cytosol of eukaryotes

Rajashekaran, Ram (2003) A novel triacylglycerol biosynthesis in the cytosol of eukaryotes. Patent Number(s) WO 03018814 A2;EP 1425398 A2;US 20030157513 A1. Patent Assignee(s) Bijam Biosciences Limited;Indian Institute of Science.


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This invention describes novel catalytically active cytosolic enzymes for triacylglycerol biosynthesis from eukary- crO otic systems. The complex from oleaginous yeast was enzymatically characterized, and was found to contain lysophosphatidic acid acyltransferase, phosphatidic acid phosphatase, diacylglycerol acyltransferase, acyl-acyl carrier protein synthetase, superoxide dismutase and acyl carrier protein. The triacylglycerol biosynthetic machinery rapidly incorporates free fatty acids as well as fatty acyl-coenzyme A into triacylglycerol and its biosynthetic intermediates. Lysophosphatidic acid acyltransferase, phosphatidic acid phosphatase and diacylglycerol acyltransferase from the complex were microsequenced. Acyl carrier protein, superoxide dismutase and diacylglycerol acyltransferase genes were cloned and expressed in bacterial system. The soluble triacylglycerol biosynthetic enzymes (lysophosphatidic acid acyltransferase, phosphatidic acid phosphatase, diacylglycerol acyltransferase) in yeast, rat adipocytes and human hepatocyte cell-line (HepG2) exist in the cytosol either as free enzymes or as a multienzyme complex.

Item Type: Patent
Department/Centre: Division of Biological Sciences > Biochemistry
Date Deposited: 25 Jul 2005
Last Modified: 19 Sep 2010 04:18
URI: http://eprints.iisc.ac.in/id/eprint/3072

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