Jagannadha, Rao A and Chakraborti, R (1989) Studies on non-specific interference due to serum in the avidin-biotin microELISA for monkey chorionic gonadotropin and a method for its elimination. In: Journal of Immunological Methods, 125 (1-2). pp. 261-264.
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Official URL: http://dx.doi.org/10.1016/0022-1759(89)90101-4
Abstract
A study has been carried out on the non-specific interference due to serum in the avidin biotin micro-ELISA for monkey chorionic gonadotropin. Results suggest that it is not due to any proteolytic activity in the serum, but immunoglobulin or associated factors interfering at the level of antigen-antibody interaction. This interference was eliminated by heating samples at 60°C for 30 min.
| Item Type: | Journal Article |
|---|---|
| Publication: | Journal of Immunological Methods |
| Publisher: | Elsevier Science |
| Additional Information: | Copyright of this article belongs to Elsevier Science. |
| Keywords: | Elisa;γ-Globulin,bovine;Gonadotrophin. |
| Department/Centre: | Division of Biological Sciences > Biochemistry |
| Date Deposited: | 16 Jul 2010 06:19 |
| Last Modified: | 16 Jul 2010 06:19 |
| URI: | http://eprints.iisc.ac.in/id/eprint/30317 |
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