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Genetic characterization of Indian type O FMD virus 3A region in context with host cell preference

Maroudam, V and Nagendrakumar, SB and Rangarajan, PN and Thiagarajan, D and Srinivasan, VA (2010) Genetic characterization of Indian type O FMD virus 3A region in context with host cell preference. In: Infection, Genetics and Evolution, 10 (5). pp. 703-709.

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Official URL: http://dx.doi.org/10.1016/j.meegid.2010.03.004


The 3A region of foot-and-mouth disease virus has been implicated in host range and virulence. For example, amino acid deletions in the porcinophilic strain (O/TAW/97) at 93-102 aa of the 153 codons long 3A protein have been recognized as the determinant of species specificity. In the present study, 18 type 0 FMDV isolates from India were adapted in different cell culture systems and the 3A sequence was analyzed. These isolates had complete 3A coding sequence (153 aa) and did not exhibit growth restriction in cells based on species of origin. The 3A region was found to be highly conserved at N-terminal half (1-75 aa) but exhibited variability or substitutions towards C-terminal region (80-153). Moreover the amino acid substitutions were more frequent in recent Indian buffalo isolates but none of the Indian isolates showed deletion in 3A protein, which may be the reason for the absence of host specificity in vitro. Further inclusive analysis of 3A region will reveal interesting facts about the variability of FMD virus 3A region in an endemic environment. (C) 2010 Elsevier B.V. All rights reserved.

Item Type: Journal Article
Publication: Infection, Genetics and Evolution
Publisher: Elsevier Science
Additional Information: Copyright of this article belongs to Elsevier Science.
Keywords: Foot-and-mouth disease virus;Serotype O;Cell culture;3A region;Genomics
Department/Centre: Division of Biological Sciences > Biochemistry
Date Deposited: 13 Jul 2010 05:48
Last Modified: 19 Sep 2010 06:11
URI: http://eprints.iisc.ac.in/id/eprint/29194

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