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Mechanism of glycine uptake by the silk glands of the silkworm Bombyx mori L.

Sridhara, S and Chitra, C (1972) Mechanism of glycine uptake by the silk glands of the silkworm Bombyx mori L. In: Insect Biochemistry, 2 (8). pp. 420-430.

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Official URL: http://dx.doi.org/10.1016/0020-1790(72)90022-4


The transport of glycine in vitro into the silk glands of the silkworm has been studied. Glycine accumulates inside the tissue to a concentration higher than that present outside, indicating an active transport mechanism. The kinetics of uptake show a biphasic curve and two apparent Km values for accumulation, 0.33 mM and 5.00 mM. The effect of inhibitors on the energy metabolism of glycine transport is inconclusive. Exchange studies indicate the existence of two pools inside the gland, one that is easily removed by exchange and osmotic shock, and the other which is not. The results obtained conform with the carrier model of Britten and McClure concerning the amino-acid pool in E. coli.

Item Type: Journal Article
Publication: Insect Biochemistry
Publisher: Elsevier Science
Additional Information: Copyright of this article belongs to Elsevier Science.
Keywords: Glycine uptake,silk glands,Bombyx mot/L.
Department/Centre: Division of Biological Sciences > Microbiology & Cell Biology
Date Deposited: 13 Jul 2010 06:54
Last Modified: 23 Oct 2018 07:38
URI: http://eprints.iisc.ac.in/id/eprint/29151

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