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Differential synthesis of proteins by T4 phage and its head protein amber mutant

Devare, SG and Padayatty, JD (1972) Differential synthesis of proteins by T4 phage and its head protein amber mutant. In: Biochemical and Biophysical Research Communications, 46 (2). pp. 628-633.

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Official URL: http://dx.doi.org/10.1016/S0006-291X(72)80186-4


There was no difference in the incorporation of S-35 label into proteins of T4 and amber B17 phage grown on Escherichia coli B. The head protein peak was absent in the polyacrylamide gel electrophoretic profile of the S-35 labeled proteins of amber B17 grown on non-permissive host, E.coli B. However, an increase of 15–70% in the synthesis of other phage proteins of amber B17 over that of T4 phage was observed. The lysozyme activity increased by two fold in amber B17 in comparison with that of T4 phage grown on E.coli B. These results imply that in the absence of head protein synthesis by amber mutant there was an increase in the synthesis of other phage proteins.

Item Type: Journal Article
Publication: Biochemical and Biophysical Research Communications
Publisher: Elsevier Science
Additional Information: Copyright of this article belongs to Elsevier Science.
Department/Centre: Division of Biological Sciences > Biochemistry
Date Deposited: 12 Jul 2010 09:14
Last Modified: 19 Sep 2010 06:10
URI: http://eprints.iisc.ac.in/id/eprint/29150

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