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Fatty acid component of vitamin A ester in sheep liver

Rao, KS and Seshadri Sastry, P (1961) Fatty acid component of vitamin A ester in sheep liver. In: Archives of Biochemistry and Biophysics, 95 (2). pp. 285-289.

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Official URL: http://dx.doi.org/10.1016/0003-9861(61)90147-3


Vitamin A, when extracted along with other lipids from sheep liver, had an E1cm.1% value of 14.4, which was raised to 45.57 on removal of the phospholipids by cold acetone. Selective hydrolysis of triglycerides by an extract of acetone-dried sheep pancreas in the presence of HgCl2 as inhibitor of vitamin A esterase, followed by chromatography through alumina gave a product with E1cm.1% value of 276. This on chromatography through magnesium oxide raised the E1cm.1, value to 601.5, representing 64% pure vitamin A ester calculated as palmitate, and the total recovery was 23% of the starting oil. The purified ester preparation, when subjected to reverse-phase chromatography on silicone-impregnated paper, gave a single ultraviolet fluorescent band. The fluorescent band on hydrolysis gave only one fatty acid. This was conclusively identified to be palmitic acid.

Item Type: Journal Article
Publication: Archives of Biochemistry and Biophysics
Publisher: Elsevier Science
Additional Information: Copyright of this article belongs to Elsevier Science.
Department/Centre: Division of Biological Sciences > Biochemistry
Date Deposited: 16 Jun 2010 07:35
Last Modified: 19 Sep 2010 06:09
URI: http://eprints.iisc.ac.in/id/eprint/28451

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