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Colorimetric determination of 2,4-dinitrophenylamino groups by sodium borohydride reduction

Ramachandran, LK (1961) Colorimetric determination of 2,4-dinitrophenylamino groups by sodium borohydride reduction. In: Analytical Chemistry, 33 (8). pp. 1074-1078.

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Official URL: http://pubs.acs.org/doi/abs/10.1021/ac60176a006


When sodium borohydride is added to aqueous solutions of 2,4-dinitrophenylamino acids and related derivatives, an intense red color is formed. Measurement of the red color, with a 420 filter, permits the determination of such compounds in concentrations of 0.01 to 0.06 μmole per ml. with a precision to 2%. The reaction is highly specific-while 2,4-dinitroaniline will react to the test, o-, m-, and p-nitroanilines, 2,4-dinitrophenyl aryl or alkyl ethers, and 2,4-dinitrophenyl-imidazole and pyrrolidine derivatives will not. Heretofore aromatic nitro groups have been considered resistant to attack by sodium borohydride. The method, as developed, is applicable to the evaluation of the degree of substitution of protein amino groups by fluorodinitrobenzene.

Item Type: Journal Article
Publication: Analytical Chemistry
Publisher: American Chemical Society
Additional Information: Copyright of this article belongs to American Chemical Society.
Department/Centre: Division of Biological Sciences > Biochemistry
Date Deposited: 23 Jun 2010 09:52
Last Modified: 19 Sep 2010 06:09
URI: http://eprints.iisc.ac.in/id/eprint/28440

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