The interaction of glycoxylate with cysteine and its application to the assay of isocitritase and of transaminases involving glyoxylate

Rao, NAN and Ramakrishnan, T (1962) The interaction of glycoxylate with cysteine and its application to the assay of isocitritase and of transaminases involving glyoxylate. In: Biochimica et Biophysica Acta, 58 (2). pp. 262-265.

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Abstract

An interesting interaction between glyoxylate and cystein takes place in phosphate buffer (pH 7.0) to form a product which is resistant to hydrolysis at ordinary temperatures. The reaction product is broken up by acid hydrolysis at elevated temperatures under controlled conditions, giving a quantitive yield of glyoxylate. Other keto acids, such as α-ketoglutarate, pyruvate and oxaloacetate, do not interact with cysteine under similar conditions. Methods based on these findings are described for(a) direct estimation of other keto acids in the presence of glyoxylate, and (b) assay of isocitritase and glyoxylate transaminase.

Item Type:	Journal Article
Publication:	Biochimica et Biophysica Acta
Publisher:	Elsevier Science
Additional Information:	Copyright of this article belongs to Elsevier Science.
Department/Centre:	Division of Biological Sciences > Microbiology & Cell Biology
Date Deposited:	23 Jun 2010 10:09
Last Modified:	26 Oct 2018 07:53
URI:	http://eprints.iisc.ac.in/id/eprint/28389

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