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A colorimetric method for determination of pyrocatechol and related substances

Nair, Madhusudanan P and Vaidyanathan, CS (1964) A colorimetric method for determination of pyrocatechol and related substances. In: Analytical Biochemistry, 7 (3). pp. 315-321.

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Official URL: http://dx.doi.org/10.1016/0003-2697(64)90136-8


A colorimetric assay for the quantitative determination of catecholic compounds was developed. The method was based on the observation that a red color was formed when nitrite was added to a solution containing pyrocatechol and sodium tungstate. Aromatic amines interfere with the reaction but this could be overcome by the addition of formaldehyde. When interfering substances are present along with pyrocatechol, it can be readily separated by paper chromatography and estimated after elution from the filter paper.

Item Type: Journal Article
Publication: Analytical Biochemistry
Publisher: Elsevier Science
Additional Information: Copyright of this article belongs to Elsevier Science.
Department/Centre: Division of Biological Sciences > Biochemistry
Date Deposited: 24 May 2010 07:18
Last Modified: 19 Sep 2010 06:07
URI: http://eprints.iisc.ac.in/id/eprint/28093

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