ePrints@IIScePrints@IISc Home | About | Browse | Latest Additions | Advanced Search | Contact | Help

Acylation of lysolecithin in the intestinal mucosa of rats

Subbaiah, PV and Sastry, PS and Ganguly, Jagannath (1970) Acylation of lysolecithin in the intestinal mucosa of rats. In: Biochemical Journal, 118 (2). pp. 241-246.

[img] PDF
ac.pdf - Published Version
Restricted to Registered users only

Download (876kB) | Request a copy
Official URL: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC117910...


1. The presence of an active acyl-CoA–lysolecithin (1-acylglycerophosphorylcholine) acyltransferase was demonstrated in rat intestinal mucosa. 2. ATP and CoA were necessary for the incorporation of free [1-14C]oleic acid into lecithin (phosphatidylcholine). 3. The reaction was about 20 times as fast with [1-14C]oleoyl-CoA as with free oleic acid, CoA and ATP. 4. With 1-acylglycerophosphorylcholine as the acceptor, both oleic acid and palmitic acid were incorporated into the β-position of lecithin; the incorporation of palmitic acid was 60% of that of oleic acid. 5. Of the various analogues of lysolecithin tested as acyl acceptors from [1-14C]oleoyl CoA, a lysolecithin with a long-chain fatty acid at the 1-position was most efficient. 6. The enzyme was mostly present in the brush-border-free particulate fraction of the intestinal mucosa. 7. Of the various tissues of rats tested for the activity, intestinal mucosa was found to be the most active, with testes, liver, kidneys and spleen following it in decreasing order.

Item Type: Journal Article
Publication: Biochemical Journal
Publisher: Portland Press
Additional Information: Copyright of this article belongs to Portland Press.
Department/Centre: Division of Biological Sciences > Biochemistry
Date Deposited: 18 May 2010 09:28
Last Modified: 19 Sep 2010 06:06
URI: http://eprints.iisc.ac.in/id/eprint/27865

Actions (login required)

View Item View Item