Disc electrophoresis of sorghum seed proteins in polyacrylamide gels

Sastry, LVS and Virupaksha, TK (1967) Disc electrophoresis of sorghum seed proteins in polyacrylamide gels. In: Analytical Biochemistry, 19 (3). pp. 505-513.

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Abstract

Electrophoretic analyses of sorghum flour protein by disc electrophoresis in polyacrylamide gels containing urea have been described. The albumin, globulin, and prolamin fractions of sorghum endosperm meal have been investigated, using pH 9.5 and 4.3 gel systems with four different buffers. Highly complex patterns were observed for all three protein fractions. It has been suggested that this method can provide a convenient tool for the analyses of seed proteins which are relatively insoluble in aqueous buffers.

Item Type:	Journal Article
Publication:	Analytical Biochemistry
Publisher:	Elsevier Science
Additional Information:	Copyright of this article belongs to Elsevier Science.
Department/Centre:	Division of Biological Sciences > Biochemistry
Date Deposited:	14 May 2010 03:48
Last Modified:	19 Sep 2010 06:06
URI:	http://eprints.iisc.ac.in/id/eprint/27821

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