Ghosh, Mridul and Surolia, A and Bachhawat, BK (1983) A simple and rapid method for quantification of hapten specific antibodies. In: Journal of Immunological Methods, 57 (1-3). pp. 295-300.
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Abstract
A sensitive and simple method for quantification of antibodies against small molecules is described using DNP-lysozyme as the enzyme conjugate. The anti-DNP antiserum was raised against DNP-bovin serum albumin conjugate. Anti-DNP antibody or its monovalent fragment (Fab) reduced the enzyme activity of DNP-lysozyme conjugate in a concentration-dependent manner. The inhibition of enzyme activity is a specific measure of the antibody and Fab content of the sample. The specificity of the reaction was assessed by reduction of antibody-induced inhibition by DNP-lysine. The ability of DNP-lysine to reduce the antibody-induced inhibition of DNP-lysozyme activity also makes possible a sensitive assay for DNP-lysine.
Item Type: | Journal Article |
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Publication: | Journal of Immunological Methods |
Publisher: | Elsevier Science |
Additional Information: | Copyright of this article belongs to Elsevier Science. |
Keywords: | Anti-hapten assay; DNP-lysine; anti-DNP antibody assay. |
Department/Centre: | Division of Biological Sciences > Molecular Biophysics Unit |
Date Deposited: | 18 May 2010 09:55 |
Last Modified: | 18 May 2010 09:55 |
URI: | http://eprints.iisc.ac.in/id/eprint/27548 |
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- A simple and rapid method for quantification of hapten specific antibodies. (deposited 18 May 2010 09:55) [Currently Displayed]
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