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Crystallization and Preliminary X-ray Studies of the Basic Lectin from Winged Bean (Psophocarpus tetragonolobus)

Sankaranarayan, R and Puri, KD and Ganesh, V and Banerjee, R and Surolia, A and Vijayan, M (1993) Crystallization and Preliminary X-ray Studies of the Basic Lectin from Winged Bean (Psophocarpus tetragonolobus). In: Journal of Molecular Biology, 229 (2). pp. 558-560.

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Official URL: http://dx.doi.org/10.1006/jmbi.1993.1056


The basic lectin from winged bean (Psophocarpus tetragonolobus) could be crystallized using polyethyleneglycol (PEG) 4000 (I), PEG 8000 (II) and 2-methylpentane-2,4-diol (MPD) (III) as precipitants. Crystal forms I and II grew in the presence of methyl-α-Image -galactopyranoside or N -acetylgalactosamine while III grew in the absence of sugar. The three forms have the same space group (P21212) and similar unit cell dimensions with two dimeric molecules in the asymmetric unit. The unit cell dimensions are a = 156·8 Å, b = 89·0 Å, c = 73·3 Å for I, a = 155·5 Å, b = 92·3 Å, c = 72·5 Å for II and a = 148·3 Å, b = 90·7 Å, c = 73·8 Å for III. The crystals, particularly those grown using PEG 8000, are suitable for high resolution X-ray analysis, which is in progress.

Item Type: Journal Article
Publication: Journal of Molecular Biology
Publisher: Elsevier Science
Additional Information: Copy right of this article belongs to Elsevier Science.
Keywords: basic winged bean lectin; X-ray analysis; legume lectins; subunit structure.
Department/Centre: Division of Biological Sciences > Molecular Biophysics Unit
Date Deposited: 05 May 2010 04:54
Last Modified: 19 Sep 2010 06:01
URI: http://eprints.iisc.ac.in/id/eprint/27517

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