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Role of 16S ribosomal RNA methylations in translation initiation in Escherichia coli

Das, Gautam and Thotala, Dinesh Kumar and Kapoor, Suman and Karunanithi, Sheelarani and Thakur, Suman S and Singh, N Sadananda and Varshney, Umesh (2008) Role of 16S ribosomal RNA methylations in translation initiation in Escherichia coli. In: EMBO Journal, 27 (6). pp. 840-851.

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Official URL: http://www.nature.com/emboj/journal/v27/n6/abs/emb...


Translation initiation from the ribosomal P-site is the specialty of the initiator tRNAs (tRNA(fMet)). Presence of the three consecutive G-C base pairs (G29-C41, G30-C40 and G31-C39) in their anticodon stems, a highly conserved feature of the initiator tRNAs across the three kingdoms of life, has been implicated in their preferential binding to the P-site. How this feature is exploited by ribosomes has remained unclear. Using a genetic screen, we have isolated an Escherichia coli strain, carrying a G122D mutation in folD, which allows initiation with the tRNA(fMet) containing mutations in one, two or all the three G-C base pairs. The strain shows a severe deficiency of methionine and S-adenosylmethionine, and lacks nucleoside methylations in rRNA. Targeted mutations in the methyltransferase genes have revealed a connection between the rRNA modifications and the fundamental process of the initiator tRNA selection by the ribosome.

Item Type: Journal Article
Publication: EMBO Journal
Publisher: Nature Publishing Group
Additional Information: Copyright of this article belongs to Nature Publishing Group.
Keywords: S-adenosyl-L-methionine;5,10 methylene tetrahydrofolate dehydrogenase;methyltransferase;modified nucleoside;folD.
Department/Centre: Division of Biological Sciences > Microbiology & Cell Biology
Date Deposited: 30 Mar 2010 09:05
Last Modified: 30 Mar 2010 09:05
URI: http://eprints.iisc.ac.in/id/eprint/26698

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