Functional characterization of lysophosphatidic acid phosphatase from Arabidopsis thaliana

Reddy, Venky Sreedhar and Rao, DK Venkata and Rajasekharan, Ram (2010) Functional characterization of lysophosphatidic acid phosphatase from Arabidopsis thaliana. In: Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids, 1801 (4). pp. 455-461.

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Abstract

Lysophosphatidic acid (LPA) acts as a signaling molecule that regulates diverse cellular processes and it can rapidly be metabolized by phosphatase and acyltransferase LPA phosphatase gene has not been identified and characterized in plants so far The BLAST search revealed that the At3g03520 is similar to phospholipase family. and distantly related to bacterial phosphatases The conserved motif. (J)4XXXNXSFD, was identified in both At3g03520 like phospholipases and acid phosphatases In silico expression analysis of At3g03520 revealed a high expression during phosphate starvation and abiotic stresses. This gene was overexpressed in Escherichia coli and shown to posses LPA specific phosphatase activity These results Suggest that this gene possibly plays a role in signal transduction and storage lipid synthesis.

Item Type:	Journal Article
Publication:	Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids
Publisher:	Elsevier Science.
Additional Information:	Copyright of this article belongs to Elsevier Science.
Keywords:	Lysophosphatidic acid phosphatase; Lipid metabolism; Phylogenetic analysis; Soluble triacylglycerol biosynthesis; Phospholipases; Conserved motif
Department/Centre:	Division of Biological Sciences > Biochemistry
Date Deposited:	29 Mar 2010 07:59
Last Modified:	19 Sep 2010 05:58
URI:	http://eprints.iisc.ac.in/id/eprint/26659

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