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Identification and characterization of DdRPB4, a subunit of Dictyostelium discoideum RNA polymerase II

Naorem, Aruna and Sadhale, Parag P (2008) Identification and characterization of DdRPB4, a subunit of Dictyostelium discoideum RNA polymerase II. In: Biochemical and Biophysical Research Communications, 377 (4). pp. 1141-1146.

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Rpb4, the fourth largest subunit of the eukaryotic RNA polymerase II (RNAPII), is required for growth at extreme temperatures and for an appropriate response to nutrient starvation in yeast. Sequence homologs of Rpb4 are found in most sequenced genomes from yeast to humans. To elucidate the role of this subunit in nutrient starvation, we chose Dictyostelium discoideum, a soil amoeba, which responds to nutrient deprivation by undergoing a complex developmental program. Here we report the identification of homolog of Saccharomyces cerevisiae RPB4 in D. discoideum. Localization and complementation studies suggest that Rpb4 is functionally conserved. DdRPB4 transcript and protein levels are developmentally regulated. Although DdRPB4 could not be deleted, overexpression revealed that the Rpb4 protein is essential for cell survival and is regulated stringently at the post-transcriptional level in D. discoideum. Thus maintaining a critical level of Rpb4 is important for this organism.

Item Type: Journal Article
Publication: Biochemical and Biophysical Research Communications
Publisher: Elsevier Science
Additional Information: Copyright of this article belongs to Elsevier Science.
Keywords: Dictyostelium discoideum;RNA polymerase II;Transcriptional regulation;Rpb4;Saccharomyces cerevisiae.
Department/Centre: Division of Biological Sciences > Microbiology & Cell Biology
Date Deposited: 23 Mar 2010 11:24
Last Modified: 19 Sep 2010 05:58
URI: http://eprints.iisc.ac.in/id/eprint/26484

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