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Evolution of sequence specificity in a restriction endonuclease by a point mutation

Saravanan, Matheshwaran and Vasu, Kommireddy and Nagaraja, Valakunja (2008) Evolution of sequence specificity in a restriction endonuclease by a point mutation. In: PNAS, 105 (30). pp. 10344-10347.

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Restriction endonucleases (REases) protect bacteria from invading foreign DNAs and are endowed with exquisite sequence specificity. REases have originated from the ancestral proteins and evolved new sequence specificities by genetic recombination, gene duplication, replication slippage, and transpositional events. They are also speculated to have evolved from nonspecific endonucleases, attaining a high degree of sequence specificity through point mutations. We describe here an example of generation of exquisitely site-specific REase from a highly-promiscuous one by a single point mutation.

Item Type: Journal Article
Publication: PNAS
Publisher: National Academy of Sciences
Additional Information: Copyright of this article belongs to National Academy of Sciences.
Department/Centre: Division of Biological Sciences > Microbiology & Cell Biology
Date Deposited: 29 Mar 2010 11:33
Last Modified: 19 Sep 2010 05:57
URI: http://eprints.iisc.ac.in/id/eprint/26294

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