ePrints@IIScePrints@IISc Home | About | Browse | Latest Additions | Advanced Search | Contact | Help

Characterization of mouse neuronal Ca2+/calmodulin kinase II inhibitor alpha

Saha, Sougata and Datta, Krishnalekha and Rangarajan, Pundi (2007) Characterization of mouse neuronal Ca2+/calmodulin kinase II inhibitor alpha. In: Brain Research, 1148 . pp. 38-42.

[img] PDF
edf.pdf - Published Version
Restricted to Registered users only

Download (250kB) | Request a copy
Official URL: http://www.sciencedirect.com/science?_ob=ArticleUR...


We have overexpressed an 8.5-kDa mouse Ca2+/calmodulin kinase II inhibitor a protein (mCaMKIIN alpha) in Escherichia coli and demonstrate that the recombinant protein is a potent inhibitor of Ca2+/calmodulin kinase 11 (CaMKII) in vitro. However, antibodies raised against recombinant mCaMKIIN alpha. react with an similar to 37-kDa protein present in mouse brain. The pattern of expression of the similar to 37-kDa protein is similar to that of mCaMKIIN alpha mRNA as both are expressed in normal but not Japanese encephalitis virus (JEV)-infected mouse brain. Subcellular localization studies indicate that the similar to 37-kDa protein is present in the post-synaptic density (PSD) where mCaMKII alpha is known to perform key regulatory functions. We conclude that the similar to 37-kDa protein identified in this study is mCaMKIIN alpha. and its localization in the PSD indicates a novel role for this protein in the regulation of neuronal CaMKII alpha. (c) 2007 Elsevier B.V. All rights reserved.

Item Type: Journal Article
Publication: Brain Research
Publisher: Elsevier Science
Additional Information: Copyright of this article belongs to Elsevier Science.
Department/Centre: Division of Biological Sciences > Biochemistry
Date Deposited: 12 Mar 2010 09:30
Last Modified: 19 Sep 2010 05:56
URI: http://eprints.iisc.ac.in/id/eprint/26140

Actions (login required)

View Item View Item