Jayabaskaran, C (1984) Immunospecific binding of tRNA precursors containing N6-(delta 2-isopentenyl) adenosine. In: Biochemistry International, 8 (2). pp. 257-64.
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Antibodies specific for N6-(delta 2-isopentenyl) adenosine (i6A) were immobilized on Sepharose and this adsorbent (Sepharose-anti-i6A) was used to selectively isolate bacteriophage T4 tRNA precursors containing i6A/ms2i6A from an unfractionated population of 32P-labeled T4 RNAs. The results showed that antibodies to i6A selectively bound only those tRNA precursors containing i6A/ms2i6A. Binding of tRNA precursors by antibody and specificity of the binding was assessed by membrane binding using 32P-labeled tRNA precursor. Binding was highly specific for i6A/ms2i6A residues in the tRNA precursors. This binding can be used to separate modified from unmodified precursor RNAs and to study the biosynthetic pathways of tRNA precursors.
| Item Type: | Journal Article |
|---|---|
| Publication: | Biochemistry International |
| Publisher: | National Center for Biotechnology Information |
| Additional Information: | Copyright of this article belongs to National Center for Biotechnology Information. |
| Department/Centre: | Division of Biological Sciences > Biochemistry |
| Date Deposited: | 24 Feb 2010 08:10 |
| Last Modified: | 24 Feb 2010 08:10 |
| URI: | http://eprints.iisc.ac.in/id/eprint/25612 |
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