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Identification and characterization of thionucleosides in the total tRNA of cucumber cotyledons

Jayabaskaran, C and Puttaraju, M (1994) Identification and characterization of thionucleosides in the total tRNA of cucumber cotyledons. In: Biochemistry & Molecular Biology International, 33 (6). pp. 1237-1247.

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Official URL: http://www.ncbi.nlm.nih.gov/pubmed/7804151


Total tRNA isolated from cucumber cotyledons grown in the presence of radioactive sulfur was analyzed for the occurrence of thionucleosides. The analysis revealed the presence of at least five thionucleosides which were identified as 5-methylaminomethyl-2-thiouridine (mnm5s2U), 2-methylthio-N6-isopentenyladenosine (ms2i6A), 2-methylthio-N6-hydroxyisopentenyladenosine (ms2io6A), 5-methyl-2-thiouridine (m5s2U) and N-[(9-beta-ribofuranosyl-2- methylthiopurine-2-yl)-carbamoyl]-threonine (ms2t6A). A comparison of relative amounts of these thionucleosides in the total tRNAs of dark-, and light-grown cotyledons shows that the relative amounts of ms2i6A, ms2io6A and ms2t6A remain unchanged whereas mnm5s2U increases with a concomitant decrease in the relative amounts of m5s2U after light treatment of dark-grown cotyledons.

Item Type: Journal Article
Publication: Biochemistry & Molecular Biology International
Publisher: National Center for Biotechnology Information
Additional Information: Copyright of this article belongs to National Center for Biotechnology Information.
Department/Centre: Division of Biological Sciences > Biochemistry
Date Deposited: 24 Feb 2010 08:15
Last Modified: 24 Feb 2010 08:15
URI: http://eprints.iisc.ac.in/id/eprint/25609

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