A glimpse into the clinical proteome of human malaria parasites Plasmodium falciparum and Plasmodium vivax

Acharya, Pragyan and Pallavi, Rani and Chandran, Syama and Chakravarti, Harshini and Middha, Sheetal and Acharya, Jyoti and Kochar, Sanjay and Kochar, Dhanpat and Subudhi, Amit and Boopathi, Arun P and Garg, Shilpi and Das, Ashis and Tatu, Utpal (2009) A glimpse into the clinical proteome of human malaria parasites Plasmodium falciparum and Plasmodium vivax. In: Proteomics - Clinical Applications, 3 (11). pp. 1314-1325.

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Abstract

Malaria causes a worldwide annual mortality of about a million people.Rapidly evolving drug-resistant species of the parasite have created a pressing need for the identification of new drug targets and vaccine candidates. By developing fractionation protocols to enrich parasites from low-parasitemia patient samples, we have carried out the first ever proteomics analysis of clinical isolates of early stages of Plasmodium falciparum (Pf) and P. vivax. Patient-derived malarial parasites were directly processed and analyzed using shotgun proteomics approach using high-sensitivity MS for protein identification. Our study revealed about 100 parasite-coded gene products that included many known drug targets such as Pf hypoxanthine guanine phosphoribosyl transferase, Pf L-lactate dehydrogenase, and Plasmepsins. In addition,our study reports the expression of several parasite proteins in clinical ring stages that have never been reported in the ring stages of the laboratory-cultivated parasite strain. This proof-of-principle study represents a noteworthy step forward in our understanding of pathways elaborated by the parasite within the malaria patient and will pave the way towards identification of new drug and vaccine targets that can aid malaria therapy.

Item Type:	Journal Article
Publication:	Proteomics - Clinical Applications
Publisher:	John Wiley and Sons
Additional Information:	Copyright of this article belongs to John Wiley and Sons.
Keywords:	Malaria;MS;Patient sample;Plasmodium.
Department/Centre:	Division of Biological Sciences > Biochemistry
Date Deposited:	10 Jan 2010 07:51
Last Modified:	24 Feb 2012 05:39
URI:	http://eprints.iisc.ac.in/id/eprint/25274

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